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source w dermatitidis 8656 wild type atcc 34100 hf1 w dermatitidis 8656 ts hyphal form mutant 50  (ATCC)


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    ATCC source w dermatitidis 8656 wild type atcc 34100 hf1 w dermatitidis 8656 ts hyphal form mutant 50
    FIG. 1. Effects of WdSTUA disruption on growth and colony morphology on YPDA at 25°C and 37°C. (A) Yeast cells were spotted (5 l) at 105, 104, 103, and 102 concentrations on YPDA and incubated at 25°C and 37°C. After 4 days, the growth at the spots was photographed without magnification, whereas the surface morphologies at the spots inoculated with the 102 yeast concentration were visualized with a dissecting microscope and also photographed. WT, wild type. (B) <t>Wild-type</t> and Wdstua1A strains cultured with shaking in YPDB at 37°C to late log phase were visualized with a compound light microscope and then photographed. Note that the Wdstua1A strain produced more hyphae that tended to be aggregated. Scale bar, 10 m (applicable to the growth in both photomicrographs).
    Source W Dermatitidis 8656 Wild Type Atcc 34100 Hf1 W Dermatitidis 8656 Ts Hyphal Form Mutant 50, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "WdStuAp, an APSES Transcription Factor, Is a Regulator of Yeast-Hyphal Transitions in Wangiella ( Exophiala ) dermatitidis"

    Article Title: WdStuAp, an APSES Transcription Factor, Is a Regulator of Yeast-Hyphal Transitions in Wangiella ( Exophiala ) dermatitidis

    Journal: Eukaryotic Cell

    doi: 10.1128/ec.00037-07

    FIG. 1. Effects of WdSTUA disruption on growth and colony morphology on YPDA at 25°C and 37°C. (A) Yeast cells were spotted (5 l) at 105, 104, 103, and 102 concentrations on YPDA and incubated at 25°C and 37°C. After 4 days, the growth at the spots was photographed without magnification, whereas the surface morphologies at the spots inoculated with the 102 yeast concentration were visualized with a dissecting microscope and also photographed. WT, wild type. (B) Wild-type and Wdstua1A strains cultured with shaking in YPDB at 37°C to late log phase were visualized with a compound light microscope and then photographed. Note that the Wdstua1A strain produced more hyphae that tended to be aggregated. Scale bar, 10 m (applicable to the growth in both photomicrographs).
    Figure Legend Snippet: FIG. 1. Effects of WdSTUA disruption on growth and colony morphology on YPDA at 25°C and 37°C. (A) Yeast cells were spotted (5 l) at 105, 104, 103, and 102 concentrations on YPDA and incubated at 25°C and 37°C. After 4 days, the growth at the spots was photographed without magnification, whereas the surface morphologies at the spots inoculated with the 102 yeast concentration were visualized with a dissecting microscope and also photographed. WT, wild type. (B) Wild-type and Wdstua1A strains cultured with shaking in YPDB at 37°C to late log phase were visualized with a compound light microscope and then photographed. Note that the Wdstua1A strain produced more hyphae that tended to be aggregated. Scale bar, 10 m (applicable to the growth in both photomicrographs).

    Techniques Used: Disruption, Incubation, Concentration Assay, Microscopy, Cell Culture, Light Microscopy, Produced

    FIG. 2. The WdSTUA deletion reduced W. dermatitidis filamentous growth on PDA at 25°C. The wild-type (WT) and Wdstua1A strains were streaked on PDA and incubated at 25°C (A) and 37°C (B). The filamentous growth at the colony edges was visualized with a compound light microscope, and the photomicrographs were taken after 4 days of incubation. Scale bar, 0.2 mm (applicable to all colonies in the figure).
    Figure Legend Snippet: FIG. 2. The WdSTUA deletion reduced W. dermatitidis filamentous growth on PDA at 25°C. The wild-type (WT) and Wdstua1A strains were streaked on PDA and incubated at 25°C (A) and 37°C (B). The filamentous growth at the colony edges was visualized with a compound light microscope, and the photomicrographs were taken after 4 days of incubation. Scale bar, 0.2 mm (applicable to all colonies in the figure).

    Techniques Used: Incubation, Light Microscopy

    FIG. 3. The WdSTUA deletion repressed aerial hyphal growth and consequently conidiogenesis. The wild-type (WT) and Wdstua1A strains were inoculated on PDA in slide cultures and incubated at 25°C. Growth at the edge of the medium and protruding into the air space between the slide and coverslip was visualized with a compound light microscope and photographed after 2 weeks of incubation. Scale bar, 10 m (applicable to all the growth in both photomicrographs). The arrow points to a cluster of conidia produced at the terminus of a conidiogenous hypha.
    Figure Legend Snippet: FIG. 3. The WdSTUA deletion repressed aerial hyphal growth and consequently conidiogenesis. The wild-type (WT) and Wdstua1A strains were inoculated on PDA in slide cultures and incubated at 25°C. Growth at the edge of the medium and protruding into the air space between the slide and coverslip was visualized with a compound light microscope and photographed after 2 weeks of incubation. Scale bar, 10 m (applicable to all the growth in both photomicrographs). The arrow points to a cluster of conidia produced at the terminus of a conidiogenous hypha.

    Techniques Used: Incubation, Light Microscopy, Produced

    FIG. 4. The Wdstua mutant was defective in invasive growth. (A) Wild-type (WT) and Wdstua1A cells were spotted on PDA and incubated at 25°C. After incubation for 8 days, colonies were visualized with a dissecting microscope and photographed. (B) The biomass above the agar was then rinsed away and the remaining, invasive growth photographed again. (C) Cross sections of the growth that invaded the agar. (D) The morphotypes in the invasive growth visualized with a light microscope. Scale bar, 10 m (applicable to the growth in both photomicrographs).
    Figure Legend Snippet: FIG. 4. The Wdstua mutant was defective in invasive growth. (A) Wild-type (WT) and Wdstua1A cells were spotted on PDA and incubated at 25°C. After incubation for 8 days, colonies were visualized with a dissecting microscope and photographed. (B) The biomass above the agar was then rinsed away and the remaining, invasive growth photographed again. (C) Cross sections of the growth that invaded the agar. (D) The morphotypes in the invasive growth visualized with a light microscope. Scale bar, 10 m (applicable to the growth in both photomicrographs).

    Techniques Used: Mutagenesis, Incubation, Microscopy, Light Microscopy

    FIG. 5. WdSTUA deletion in the Hf1 strain inhibited filamentous growth on PDA. Wild-type (WT), Wdstua1A, Hf1, and Hf1 Wdstua1 strains were grown on YPDA at 37°C for 6 days (A) and on PDA at 25°C for 6 days (B). The Hf1 and Hf1 Wdstua1 strains were also inoculated on PDA for slide culture at 25°C. After 2 weeks, conidiophores and conidia were visualized with a light microscope and photographed (C). Scale bars, 10 m (applicable to the growth in both photomicrographs). The arrows point to clusters of conidia produced at the termini of conidiogenous hyphae.
    Figure Legend Snippet: FIG. 5. WdSTUA deletion in the Hf1 strain inhibited filamentous growth on PDA. Wild-type (WT), Wdstua1A, Hf1, and Hf1 Wdstua1 strains were grown on YPDA at 37°C for 6 days (A) and on PDA at 25°C for 6 days (B). The Hf1 and Hf1 Wdstua1 strains were also inoculated on PDA for slide culture at 25°C. After 2 weeks, conidiophores and conidia were visualized with a light microscope and photographed (C). Scale bars, 10 m (applicable to the growth in both photomicrographs). The arrows point to clusters of conidia produced at the termini of conidiogenous hyphae.

    Techniques Used: Light Microscopy, Produced

    Related Articles

    Mutagenesis:

    Article Title: WdStuAp, an APSES Transcription Factor, Is a Regulator of Yeast-Hyphal Transitions in Wangiella ( Exophiala ) dermatitidis
    Article Snippet: .. Strains used in this research Strain Parent strain Genotypea Reference(s) or source W. dermatitidis 8656 Wild type ATCC 34100 Hf1 W. dermatitidis 8656 ts hyphal-form mutant 50, 53 Wdstua 1A W. dermatitidis 8656 Wdstua::hph This study Wdstua 1B W. dermatitidis 8656 Wdstua::hph This study Wdstua 2 W. dermatitidis 8656 Wdstua::sur This study Hf1 Wdstua 1 Hf1 Hf1 Wdstua::hph This study Wdpks1 -1 W. dermatitidis 8656 WdSTUAWt Wdpks1::pYEX303-hph This study Wdpks1 WdSTUA-1 W. dermatitidis 8656 WdSTUAWt Wdpks1::pYEX303-hph-WdSTUAEct This study Wdpks1 Wdstua -1 Wdstua 2 Wdstua::sur Wdpks1::pYEX303-hph This study Wdpks1 Wdstua WdSTUA-1 Wdstua 2 Wdstua::sur Wdpks1::pYEX303-hph-WdSTUAEct This study S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 35 MR12-pYES2 S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 (pYES2) This study MR12-pYES2WdSTUA S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 (pYES2-WdSTUA) This study a WdSTUAWt designates the wild-type endogenous gene; WdSTUAEct designates the gene ectopically integrated in the WdPKS1 locus. on July 4, 2015 by U niv of M assachusetts A m herst http://ec.asm .org/ D ow nloaded from prior to incubation over water in a closed petri dish. .. Colony morphology pictures were taken with a Canon Powershot G3 camera attached to an Olympus model SZ-III dissecting microscope fitted with a ScopeTronix Maxview Plus adapter.

    Incubation:

    Article Title: WdStuAp, an APSES Transcription Factor, Is a Regulator of Yeast-Hyphal Transitions in Wangiella ( Exophiala ) dermatitidis
    Article Snippet: .. Strains used in this research Strain Parent strain Genotypea Reference(s) or source W. dermatitidis 8656 Wild type ATCC 34100 Hf1 W. dermatitidis 8656 ts hyphal-form mutant 50, 53 Wdstua 1A W. dermatitidis 8656 Wdstua::hph This study Wdstua 1B W. dermatitidis 8656 Wdstua::hph This study Wdstua 2 W. dermatitidis 8656 Wdstua::sur This study Hf1 Wdstua 1 Hf1 Hf1 Wdstua::hph This study Wdpks1 -1 W. dermatitidis 8656 WdSTUAWt Wdpks1::pYEX303-hph This study Wdpks1 WdSTUA-1 W. dermatitidis 8656 WdSTUAWt Wdpks1::pYEX303-hph-WdSTUAEct This study Wdpks1 Wdstua -1 Wdstua 2 Wdstua::sur Wdpks1::pYEX303-hph This study Wdpks1 Wdstua WdSTUA-1 Wdstua 2 Wdstua::sur Wdpks1::pYEX303-hph-WdSTUAEct This study S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 35 MR12-pYES2 S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 (pYES2) This study MR12-pYES2WdSTUA S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 (pYES2-WdSTUA) This study a WdSTUAWt designates the wild-type endogenous gene; WdSTUAEct designates the gene ectopically integrated in the WdPKS1 locus. on July 4, 2015 by U niv of M assachusetts A m herst http://ec.asm .org/ D ow nloaded from prior to incubation over water in a closed petri dish. .. Colony morphology pictures were taken with a Canon Powershot G3 camera attached to an Olympus model SZ-III dissecting microscope fitted with a ScopeTronix Maxview Plus adapter.



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    source w dermatitidis 8656 wild type atcc 34100 hf1 w dermatitidis 8656 ts hyphal form mutant 50  (ATCC)
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    ATCC source w dermatitidis 8656 wild type atcc 34100 hf1 w dermatitidis 8656 ts hyphal form mutant 50
    FIG. 1. Effects of WdSTUA disruption on growth and colony morphology on YPDA at 25°C and 37°C. (A) Yeast cells were spotted (5 l) at 105, 104, 103, and 102 concentrations on YPDA and incubated at 25°C and 37°C. After 4 days, the growth at the spots was photographed without magnification, whereas the surface morphologies at the spots inoculated with the 102 yeast concentration were visualized with a dissecting microscope and also photographed. WT, wild type. (B) <t>Wild-type</t> and Wdstua1A strains cultured with shaking in YPDB at 37°C to late log phase were visualized with a compound light microscope and then photographed. Note that the Wdstua1A strain produced more hyphae that tended to be aggregated. Scale bar, 10 m (applicable to the growth in both photomicrographs).
    Source W Dermatitidis 8656 Wild Type Atcc 34100 Hf1 W Dermatitidis 8656 Ts Hyphal Form Mutant 50, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/source+w+dermatitidis+8656+wild+type+atcc+34100+hf1+w+dermatitidis+8656+ts+hyphal+form+mutant+50/Exophiala+dermatitidis%3B+8656/10__1128_slash_ec__00037___07-72-11-17
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    source w dermatitidis 8656 wild type atcc 34100 hf1 w dermatitidis 8656 ts hyphal form mutant 50 - by Bioz Stars, 2026-10
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    FIG. 1. Effects of WdSTUA disruption on growth and colony morphology on YPDA at 25°C and 37°C. (A) Yeast cells were spotted (5 l) at 105, 104, 103, and 102 concentrations on YPDA and incubated at 25°C and 37°C. After 4 days, the growth at the spots was photographed without magnification, whereas the surface morphologies at the spots inoculated with the 102 yeast concentration were visualized with a dissecting microscope and also photographed. WT, wild type. (B) Wild-type and Wdstua1A strains cultured with shaking in YPDB at 37°C to late log phase were visualized with a compound light microscope and then photographed. Note that the Wdstua1A strain produced more hyphae that tended to be aggregated. Scale bar, 10 m (applicable to the growth in both photomicrographs).

    Journal: Eukaryotic Cell

    Article Title: WdStuAp, an APSES Transcription Factor, Is a Regulator of Yeast-Hyphal Transitions in Wangiella ( Exophiala ) dermatitidis

    doi: 10.1128/ec.00037-07

    Figure Lengend Snippet: FIG. 1. Effects of WdSTUA disruption on growth and colony morphology on YPDA at 25°C and 37°C. (A) Yeast cells were spotted (5 l) at 105, 104, 103, and 102 concentrations on YPDA and incubated at 25°C and 37°C. After 4 days, the growth at the spots was photographed without magnification, whereas the surface morphologies at the spots inoculated with the 102 yeast concentration were visualized with a dissecting microscope and also photographed. WT, wild type. (B) Wild-type and Wdstua1A strains cultured with shaking in YPDB at 37°C to late log phase were visualized with a compound light microscope and then photographed. Note that the Wdstua1A strain produced more hyphae that tended to be aggregated. Scale bar, 10 m (applicable to the growth in both photomicrographs).

    Article Snippet: Strains used in this research Strain Parent strain Genotypea Reference(s) or source W. dermatitidis 8656 Wild type ATCC 34100 Hf1 W. dermatitidis 8656 ts hyphal-form mutant 50, 53 Wdstua 1A W. dermatitidis 8656 Wdstua::hph This study Wdstua 1B W. dermatitidis 8656 Wdstua::hph This study Wdstua 2 W. dermatitidis 8656 Wdstua::sur This study Hf1 Wdstua 1 Hf1 Hf1 Wdstua::hph This study Wdpks1 -1 W. dermatitidis 8656 WdSTUAWt Wdpks1::pYEX303-hph This study Wdpks1 WdSTUA-1 W. dermatitidis 8656 WdSTUAWt Wdpks1::pYEX303-hph-WdSTUAEct This study Wdpks1 Wdstua -1 Wdstua 2 Wdstua::sur Wdpks1::pYEX303-hph This study Wdpks1 Wdstua WdSTUA-1 Wdstua 2 Wdstua::sur Wdpks1::pYEX303-hph-WdSTUAEct This study S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 35 MR12-pYES2 S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 (pYES2) This study MR12-pYES2WdSTUA S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 (pYES2-WdSTUA) This study a WdSTUAWt designates the wild-type endogenous gene; WdSTUAEct designates the gene ectopically integrated in the WdPKS1 locus. on July 4, 2015 by U niv of M assachusetts A m herst http://ec.asm .org/ D ow nloaded from prior to incubation over water in a closed petri dish.

    Techniques: Disruption, Incubation, Concentration Assay, Microscopy, Cell Culture, Light Microscopy, Produced

    FIG. 2. The WdSTUA deletion reduced W. dermatitidis filamentous growth on PDA at 25°C. The wild-type (WT) and Wdstua1A strains were streaked on PDA and incubated at 25°C (A) and 37°C (B). The filamentous growth at the colony edges was visualized with a compound light microscope, and the photomicrographs were taken after 4 days of incubation. Scale bar, 0.2 mm (applicable to all colonies in the figure).

    Journal: Eukaryotic Cell

    Article Title: WdStuAp, an APSES Transcription Factor, Is a Regulator of Yeast-Hyphal Transitions in Wangiella ( Exophiala ) dermatitidis

    doi: 10.1128/ec.00037-07

    Figure Lengend Snippet: FIG. 2. The WdSTUA deletion reduced W. dermatitidis filamentous growth on PDA at 25°C. The wild-type (WT) and Wdstua1A strains were streaked on PDA and incubated at 25°C (A) and 37°C (B). The filamentous growth at the colony edges was visualized with a compound light microscope, and the photomicrographs were taken after 4 days of incubation. Scale bar, 0.2 mm (applicable to all colonies in the figure).

    Article Snippet: Strains used in this research Strain Parent strain Genotypea Reference(s) or source W. dermatitidis 8656 Wild type ATCC 34100 Hf1 W. dermatitidis 8656 ts hyphal-form mutant 50, 53 Wdstua 1A W. dermatitidis 8656 Wdstua::hph This study Wdstua 1B W. dermatitidis 8656 Wdstua::hph This study Wdstua 2 W. dermatitidis 8656 Wdstua::sur This study Hf1 Wdstua 1 Hf1 Hf1 Wdstua::hph This study Wdpks1 -1 W. dermatitidis 8656 WdSTUAWt Wdpks1::pYEX303-hph This study Wdpks1 WdSTUA-1 W. dermatitidis 8656 WdSTUAWt Wdpks1::pYEX303-hph-WdSTUAEct This study Wdpks1 Wdstua -1 Wdstua 2 Wdstua::sur Wdpks1::pYEX303-hph This study Wdpks1 Wdstua WdSTUA-1 Wdstua 2 Wdstua::sur Wdpks1::pYEX303-hph-WdSTUAEct This study S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 35 MR12-pYES2 S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 (pYES2) This study MR12-pYES2WdSTUA S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 (pYES2-WdSTUA) This study a WdSTUAWt designates the wild-type endogenous gene; WdSTUAEct designates the gene ectopically integrated in the WdPKS1 locus. on July 4, 2015 by U niv of M assachusetts A m herst http://ec.asm .org/ D ow nloaded from prior to incubation over water in a closed petri dish.

    Techniques: Incubation, Light Microscopy

    FIG. 3. The WdSTUA deletion repressed aerial hyphal growth and consequently conidiogenesis. The wild-type (WT) and Wdstua1A strains were inoculated on PDA in slide cultures and incubated at 25°C. Growth at the edge of the medium and protruding into the air space between the slide and coverslip was visualized with a compound light microscope and photographed after 2 weeks of incubation. Scale bar, 10 m (applicable to all the growth in both photomicrographs). The arrow points to a cluster of conidia produced at the terminus of a conidiogenous hypha.

    Journal: Eukaryotic Cell

    Article Title: WdStuAp, an APSES Transcription Factor, Is a Regulator of Yeast-Hyphal Transitions in Wangiella ( Exophiala ) dermatitidis

    doi: 10.1128/ec.00037-07

    Figure Lengend Snippet: FIG. 3. The WdSTUA deletion repressed aerial hyphal growth and consequently conidiogenesis. The wild-type (WT) and Wdstua1A strains were inoculated on PDA in slide cultures and incubated at 25°C. Growth at the edge of the medium and protruding into the air space between the slide and coverslip was visualized with a compound light microscope and photographed after 2 weeks of incubation. Scale bar, 10 m (applicable to all the growth in both photomicrographs). The arrow points to a cluster of conidia produced at the terminus of a conidiogenous hypha.

    Article Snippet: Strains used in this research Strain Parent strain Genotypea Reference(s) or source W. dermatitidis 8656 Wild type ATCC 34100 Hf1 W. dermatitidis 8656 ts hyphal-form mutant 50, 53 Wdstua 1A W. dermatitidis 8656 Wdstua::hph This study Wdstua 1B W. dermatitidis 8656 Wdstua::hph This study Wdstua 2 W. dermatitidis 8656 Wdstua::sur This study Hf1 Wdstua 1 Hf1 Hf1 Wdstua::hph This study Wdpks1 -1 W. dermatitidis 8656 WdSTUAWt Wdpks1::pYEX303-hph This study Wdpks1 WdSTUA-1 W. dermatitidis 8656 WdSTUAWt Wdpks1::pYEX303-hph-WdSTUAEct This study Wdpks1 Wdstua -1 Wdstua 2 Wdstua::sur Wdpks1::pYEX303-hph This study Wdpks1 Wdstua WdSTUA-1 Wdstua 2 Wdstua::sur Wdpks1::pYEX303-hph-WdSTUAEct This study S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 35 MR12-pYES2 S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 (pYES2) This study MR12-pYES2WdSTUA S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 (pYES2-WdSTUA) This study a WdSTUAWt designates the wild-type endogenous gene; WdSTUAEct designates the gene ectopically integrated in the WdPKS1 locus. on July 4, 2015 by U niv of M assachusetts A m herst http://ec.asm .org/ D ow nloaded from prior to incubation over water in a closed petri dish.

    Techniques: Incubation, Light Microscopy, Produced

    FIG. 4. The Wdstua mutant was defective in invasive growth. (A) Wild-type (WT) and Wdstua1A cells were spotted on PDA and incubated at 25°C. After incubation for 8 days, colonies were visualized with a dissecting microscope and photographed. (B) The biomass above the agar was then rinsed away and the remaining, invasive growth photographed again. (C) Cross sections of the growth that invaded the agar. (D) The morphotypes in the invasive growth visualized with a light microscope. Scale bar, 10 m (applicable to the growth in both photomicrographs).

    Journal: Eukaryotic Cell

    Article Title: WdStuAp, an APSES Transcription Factor, Is a Regulator of Yeast-Hyphal Transitions in Wangiella ( Exophiala ) dermatitidis

    doi: 10.1128/ec.00037-07

    Figure Lengend Snippet: FIG. 4. The Wdstua mutant was defective in invasive growth. (A) Wild-type (WT) and Wdstua1A cells were spotted on PDA and incubated at 25°C. After incubation for 8 days, colonies were visualized with a dissecting microscope and photographed. (B) The biomass above the agar was then rinsed away and the remaining, invasive growth photographed again. (C) Cross sections of the growth that invaded the agar. (D) The morphotypes in the invasive growth visualized with a light microscope. Scale bar, 10 m (applicable to the growth in both photomicrographs).

    Article Snippet: Strains used in this research Strain Parent strain Genotypea Reference(s) or source W. dermatitidis 8656 Wild type ATCC 34100 Hf1 W. dermatitidis 8656 ts hyphal-form mutant 50, 53 Wdstua 1A W. dermatitidis 8656 Wdstua::hph This study Wdstua 1B W. dermatitidis 8656 Wdstua::hph This study Wdstua 2 W. dermatitidis 8656 Wdstua::sur This study Hf1 Wdstua 1 Hf1 Hf1 Wdstua::hph This study Wdpks1 -1 W. dermatitidis 8656 WdSTUAWt Wdpks1::pYEX303-hph This study Wdpks1 WdSTUA-1 W. dermatitidis 8656 WdSTUAWt Wdpks1::pYEX303-hph-WdSTUAEct This study Wdpks1 Wdstua -1 Wdstua 2 Wdstua::sur Wdpks1::pYEX303-hph This study Wdpks1 Wdstua WdSTUA-1 Wdstua 2 Wdstua::sur Wdpks1::pYEX303-hph-WdSTUAEct This study S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 35 MR12-pYES2 S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 (pYES2) This study MR12-pYES2WdSTUA S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 (pYES2-WdSTUA) This study a WdSTUAWt designates the wild-type endogenous gene; WdSTUAEct designates the gene ectopically integrated in the WdPKS1 locus. on July 4, 2015 by U niv of M assachusetts A m herst http://ec.asm .org/ D ow nloaded from prior to incubation over water in a closed petri dish.

    Techniques: Mutagenesis, Incubation, Microscopy, Light Microscopy

    FIG. 5. WdSTUA deletion in the Hf1 strain inhibited filamentous growth on PDA. Wild-type (WT), Wdstua1A, Hf1, and Hf1 Wdstua1 strains were grown on YPDA at 37°C for 6 days (A) and on PDA at 25°C for 6 days (B). The Hf1 and Hf1 Wdstua1 strains were also inoculated on PDA for slide culture at 25°C. After 2 weeks, conidiophores and conidia were visualized with a light microscope and photographed (C). Scale bars, 10 m (applicable to the growth in both photomicrographs). The arrows point to clusters of conidia produced at the termini of conidiogenous hyphae.

    Journal: Eukaryotic Cell

    Article Title: WdStuAp, an APSES Transcription Factor, Is a Regulator of Yeast-Hyphal Transitions in Wangiella ( Exophiala ) dermatitidis

    doi: 10.1128/ec.00037-07

    Figure Lengend Snippet: FIG. 5. WdSTUA deletion in the Hf1 strain inhibited filamentous growth on PDA. Wild-type (WT), Wdstua1A, Hf1, and Hf1 Wdstua1 strains were grown on YPDA at 37°C for 6 days (A) and on PDA at 25°C for 6 days (B). The Hf1 and Hf1 Wdstua1 strains were also inoculated on PDA for slide culture at 25°C. After 2 weeks, conidiophores and conidia were visualized with a light microscope and photographed (C). Scale bars, 10 m (applicable to the growth in both photomicrographs). The arrows point to clusters of conidia produced at the termini of conidiogenous hyphae.

    Article Snippet: Strains used in this research Strain Parent strain Genotypea Reference(s) or source W. dermatitidis 8656 Wild type ATCC 34100 Hf1 W. dermatitidis 8656 ts hyphal-form mutant 50, 53 Wdstua 1A W. dermatitidis 8656 Wdstua::hph This study Wdstua 1B W. dermatitidis 8656 Wdstua::hph This study Wdstua 2 W. dermatitidis 8656 Wdstua::sur This study Hf1 Wdstua 1 Hf1 Hf1 Wdstua::hph This study Wdpks1 -1 W. dermatitidis 8656 WdSTUAWt Wdpks1::pYEX303-hph This study Wdpks1 WdSTUA-1 W. dermatitidis 8656 WdSTUAWt Wdpks1::pYEX303-hph-WdSTUAEct This study Wdpks1 Wdstua -1 Wdstua 2 Wdstua::sur Wdpks1::pYEX303-hph This study Wdpks1 Wdstua WdSTUA-1 Wdstua 2 Wdstua::sur Wdpks1::pYEX303-hph-WdSTUAEct This study S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 35 MR12-pYES2 S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 (pYES2) This study MR12-pYES2WdSTUA S. cerevisiae MR12 MATa/ leu2::hisG/LEU2::pFLO11::lacZ::leu-2::hisG ura3-52/ura3-52 (pYES2-WdSTUA) This study a WdSTUAWt designates the wild-type endogenous gene; WdSTUAEct designates the gene ectopically integrated in the WdPKS1 locus. on July 4, 2015 by U niv of M assachusetts A m herst http://ec.asm .org/ D ow nloaded from prior to incubation over water in a closed petri dish.

    Techniques: Light Microscopy, Produced